电器官还原并烷化的乙酰胆碱酯酶(RA-A ChE)的溴化氰裂解肽或又经胃蛋白酶切割的肽与抗RA-AChE单克隆抗体E9、F6及F12仍有抗原抗体反应。经溴化氰及胰蛋白酶割的肽与3者反应消失。氧化破坏RA-AChE糖侧链、溴化氰裂解的RA-A ChE又经酶切除糖侧链后,与3者反应无变化。E9、F6及F12所识别的抗原决定簇是多肽型,不是多糖型。 Electro-organo-reduced and alkylated acetylcholinesterase (RA-A ChE) cyanogen bromide or pepsin peptide was still antigen-antibody reactive with anti-RA-AChE monoclonal antibodies E9, F6 and F12. The cyanogen bromide and tryptic peptide cleavage and 3 disappeared. The oxidative destruction of the RA-AChE sugar side chain, cyanogen bromide cleavage of RA-A ChE enzymatic excision of the sugar side chain, with 3 no change in response. The epitopes recognized by E9, F6 and F12 are polypeptide types, not polysaccharide types.