论文部分内容阅读
目的 探讨胰岛素样生长因子 Ⅱ (IGF Ⅱ )在人早孕期绒毛和蜕膜中的表达及IGF Ⅱ与ER、PR表达之间的相互关系。方法 采用单克隆抗体SP免疫组化法测定IGF Ⅱ、ER、PR在人早孕期绒毛、蜕膜中的表达 ,并用组织学积分法进行半定量分析和相关分析。结果 IGF Ⅱ在绒毛细胞滋养细胞、合体滋养细胞和蜕膜间质细胞中均有表达 ,绒毛中的表达高于蜕膜。蜕膜中ER、PR的表达呈正相关 (P <0 0 1)。而IGF Ⅱ与ER、PR之间无显著相关性 (P >0 0 5 )。结论 推测IGF Ⅱ与促进母体蜕膜和胚胎的生长发育有关 ,ER、PR的平衡有利于早孕期蜕膜的正常发育
Objective To investigate the expression of insulin-like growth factor Ⅱ (IGF Ⅱ) in human chorionic villi and decidua in early pregnancy and the relationship between IGF Ⅱ, ER and PR expression. Methods The expression of IGF Ⅱ, ER and PR in human chorionic villi and decidua were detected by SP immunohistochemical method. Semi-quantitative analysis and correlation analysis were performed by histological integration method. Results IGF Ⅱ was expressed in villus trophoblasts, syncytiotrophoblasts and decidua interstitial cells, and the expression in the villi was higher than that in the decidua. The expression of ER and PR in decidua was positively correlated (P <0.01). There was no significant correlation between IGF Ⅱ and ER, PR (P> 0.05). Conclusions It is speculated that IGF Ⅱ is related to the growth and development of decidua and embryo of maternal body. The balance of ER and PR is beneficial to the normal development of decidua in early pregnancy