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目的:观察左、右归丸及其拆方对去卵巢大鼠肾脏TGFβ1/Smad1基因与蛋白表达的影响,并探讨补肾法对去卵巢模型大鼠肾脏TGFβ1/Smad1通路的影响。方法:将90只SD雌性大鼠随机分为空白组、假手术组、模型组、补佳乐组、左归丸组、右归丸组、共同药组、滋阴药组、补阳药组。除假手术、空白组外均进行双侧卵巢切除。经12周治疗分别以Q-PCR法、Western blot法检测TGFβ1、TβRⅠ、TβRⅡ、Smad1在肾脏中基因和蛋白的表达。结果:与空白组相比,除假手术组外,TGFβ1、TβRⅠ、TβRⅡ、Smad1 mRNA及蛋白表达降低(P<0.01);与模型组比较,各用药组TGFβ1、TβRⅠ、TβRⅡ、Smad1 mRNA及蛋白表达升高(P<0.01);与补佳乐组比较,各用药组TGFβ1、TβRⅠ、TβRⅡ、Smad1表达低,其中左归丸组表达较高。结论:左、右归丸及其拆方可影响肾脏中TGFβ1/Smad1基因与蛋白的表达,其中以左归丸改变最为显著。
Objective: To observe the effects of Zuogui Gui Wan and its decomposed formulas on the expression of TGFβ1 / Smad1 gene and protein in the kidney of ovariectomized rats and to explore the effect of tonifying kidney on TGFβ1 / Smad1 pathway in the kidney of ovariectomized rats. Methods: Ninety SD female rats were randomly divided into blank group, sham operation group, model group, Bu Jiale group, Zuoguiwan group, Youguiwan group, common medicine group, Ziyin drug group, . In addition to sham surgery, both sides of the blank group were ovariectomized. The expression of TGFβ1, TβRⅠ, TβRⅡ and Smad1 in kidney were detected by Q-PCR and Western blot respectively after 12 weeks treatment. Results: Compared with the blank group, the mRNA and protein expressions of TGFβ1, TβRⅠ, TβRⅡ and Smad1 were decreased (P <0.01) except the sham operation group. Compared with the model group, the expressions of TGFβ1, TβRⅠ, TβRⅡ, Smad1 mRNA and protein The expression of TGFβ1, TβRⅠ, TβRⅡ and Smad1 in each treatment group was lower than that in the nocturnal group, especially in Zuoguiwan group. Conclusion: Zuogui Gui Wan and its disassembled formulas can affect the expression of TGFβ1 / Smad1 gene and protein in kidney, of which Zuo Gui Wan is the most obvious change.