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通常认为釉质表面未被破坏,在其内部没有细菌存在。然而,一些研究发现在早期龋齿周围的釉质内,甚至在健康牙釉质内有细菌。作者曾在鼠及人第三磨牙釉质表面观察到了细菌。本文作者用改良技术,对侵入人牙釉质的细菌做了研究。采用不同类型成人牙23颗,拔牙后放入纯酒精贮存。用水彻底刷洗有白斑龋变的光滑面,然后将其取下。样本在120℃灭菌15分钟。将其余面镀膜,釉质表面覆有氰基丙烯酸酯。与外表面垂直,从白色龋变区断开,获得断面。在所获的2个断面的龋变处
The enamel surface is generally considered undamaged, with no bacteria present in it. However, some studies have found that there are bacteria in the enamel around the early caries, even in healthy enamel. The authors observed bacteria on the surface of the third molars in rats and humans. The authors used improved techniques to investigate bacteria that invade human enamel. Using different types of adult teeth 23, after tooth extraction into pure alcohol storage. Thoroughly brush the smooth surface of the white caries with water and remove it. Samples were sterilized at 120 ° C for 15 minutes. The remaining surface coating, enamel covered with cyanoacrylate. Perpendicular to the outer surface, disconnected from the white caries area, obtaining a cross-section. In the two sections of caries obtained