采用子房注射法将构建在植物表达载体 p BI1 2 1 .1上的 Bt杀虫基因导入棉花品种石远 3 2 1、中植3 72、82 2和辽棉 90 0 1中 ,获得 8株转基因植株。经温室抗虫性鉴定 ,8株转基因植株对棉铃虫都表现出较好的抗性。同时对这 8棵棉株叶片进行的 PCR检测结果证明 Bt基因已经整合进植物基因组中 The Bt insecticidal gene, constructed on the plant expression vector pBI21.1, was introduced into cotton variety Shi Yuan3,21, Zhongban3 72,82,2 and Liaolian0901 using ovary injection to obtain 8 strains Transgenic plants. The greenhouse insect resistance identification, 8 transgenic plants showed good resistance to cotton bollworm. At the same time, the PCR results of the leaves of the eight cotton plants proved that the Bt gene has been integrated into the plant genome