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比较了细胞松弛素B(CB)和 6 -二甲基氨基嘌呤 (6 -DMAP)通过抑制受精卵极体释放的方法批量诱导三倍体长牡蛎的养殖效果。长牡蛎卵子在 2 5℃的海水中受精 ,2 0～ 30min后 ,开始用浓度为 0 .5mg·L-1的CB处理 ,持续 18～ 2 2min ,受精卵处理密度为 4.0～ 4.5× 10 7个·L-1,三倍体产率为 6 5 .2 %～ 70 .1% ,面盘幼虫孵化率为 12 .3%～ 14.5 % ,诱导效率指数为 0 .0 9。 6 -DMAP的使用浓度为 40 0～ 42 0 μmol·L-1,受精卵处理密度为 3.0～ 3 .5× 10 7个·L-1,授精水温、处理起始和持续时间等与CB方法相同 ,三倍体产率为 5 8.7%～ 6 5 .4% ,面盘幼虫孵化率为 5 2 .1%～ 5 5 .4% ,诱导效率指数为 0 .32。两种方法的采苗率基本相同 ,采苗器为基质较硬的栉孔扇贝贝壳。海区养殖采用浮筏夹苗吊养技术 ,两种方法诱导的三倍体牡蛎养殖性状没有明显差别。通过比较CB和 6 -DMAP两种诱导方法及三倍体的养殖效果表明 ,后者具有更好的应用性。
The effects of cytochalasin B (CB) and 6 - DMAP on the production of triploid oyster were compared by inhibiting the release of the polar body of the fertilized egg. The eggs of oyster oyster were fertilized in seawater at 25 ℃. After 20-30 minutes, the eggs of oyster oyster were initially treated with CB at 0.5 mg · L -1 for 18-2 min and the fertilized eggs were treated at 4.0-4.5 × 10 7 L-1, the triploid yield was 65.2% -70.1%, the hatching rate of faceplate larvae was 12.3% -14.5%, and the induction efficiency index was 0.09. The concentration of 6-DMAP was 40 0 ~ 42 0 μmol·L-1, the fertilized egg treatment density was 3.0-3.5 × 10 7 · L-1, the temperature of fertilization, the initiation and the duration of treatment, Similarly, the triploid yield was 57.7% ~ 65.4%, the hatching rate of faceplate larva was 52.2% ~ 55.4%, and the induction efficiency index was 0.32. The seedling acquisition rate of the two methods is basically the same, the harvester is the harder substrate of scallop shell. Aquaculture using floating raft sandwich seedlings hanging culture, the two methods induced triploid oyster farming traits no significant difference. By comparing CB and 6-DMAP two induction methods and triploid breeding results show that the latter has better applicability.