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目的:探讨不同浓度及作用时间的氯化锶(SrCl2)对小鼠卵母细胞孤雌激活胚胎中出现类原核数量及发育潜能的影响,建立能模拟精子效应的小鼠孤雌的激活体系。方法:分别用5 mmol/L、10 mmol/L、15 mmol/L、20 mmol/L浓度的SrCl2激活小鼠卵母细胞,观察激活胚的类原核数量及发育潜能,然后在最佳刺激浓度的SrCl2的激活液中分别处理4 h、6 h、8 h,观察类原核数目及形态,分析SrCl2浓度对小鼠卵母细胞激活效果的影响。结果:hCG注射后18 h取小鼠卵母细胞,在10 mmol/L SrCl2浓度下激活6 h,出现双原核(2PN)百分比显著高于其它浓度SrCl2处理组(P<0.01),且与小鼠体外受精6 h后的2PN率及囊胚形成率无统计学差异(P>0.05)。结论:10 mmol/L SrCl2条件下激活小鼠卵子6 h产生的孤雌胚胎中出现2PN率及发育潜能最高,提示10 mmol/L SrCl2激活处理6 h可以模拟精子对卵子的激活条件。
OBJECTIVE: To investigate the effect of different concentrations and duration of SrCl2 on the number of prokaryotic nucleoplasm and the developmental potential in parthenogenetic embryos of mouse oocytes, and to establish a mouse parthenogenetic activation system that can simulate the sperm effect. Methods: The mouse oocytes were activated with 5 mmol / L, 10 mmol / L, 15 mmol / L and 20 mmol / L SrCl2 respectively to observe the number of prokaryotic nuclei and developmental potential of activated embryos. Of SrCl2 for 4 h, 6 h, 8 h, respectively. The number and morphology of prokaryotic prokaryotic cells were observed and the effect of SrCl2 concentration on activation of mouse oocytes was analyzed. Results: The mouse oocytes were harvested at 18 h after injection of hCG and activated at 10 mmol / L SrCl2 for 6 h. The percentage of 2PN was significantly higher than that of SrCl2 (P <0.01) There was no significant difference in 2PN rate and blastocyst formation rate after 6 h in vitro fertilization (P> 0.05). CONCLUSION: The 2PN rate and developmental potential of parthenogenetic embryos produced by mouse ovum activation for 6 h under the conditions of 10 mmol / L SrCl2 are the highest, suggesting that activation of 10-20 mM SrCl2 for 6 h can simulate the activation of sperm on the ovum.