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目的:建立HPLC同时测定牻牛儿苗中没食子酸,原儿茶酸,柯里拉京,鞣花酸的含量测定方法。方法:采用RP-HPLC,AgilentTC-C18色谱柱(4.6 mm×250 mm,5μm),0.4%磷酸水溶液-甲醇为流动相梯度洗脱,流速0.8 mL.min-1,检测波长259 nm,柱温30℃。结果:没食子酸,原儿茶酸,柯里拉京,鞣花酸的线性范围为0.059～2.360 g.L-1(r=0.999 6),0.017～0.672 g.L-1(r=0.999 9),0.351～14.04 g.L-1(r=0.999 9),0.151～6.040 g.L-1(r=0.999 8)。平均回收率(n=3)分别为99.45(RSD 1.5%),98.65%(RSD 1.7%),100.3%(RSD 2.0%),98.90%(RSD 1.2%)。结论:该含量测定的方法快速,准确,可靠,重复性好,可为药材的质量控制提供依据。
OBJECTIVE: To establish a method for the simultaneous determination of gallic acid, protocatechuic acid, corilagin and ellagic acid in Goat’s milk seedling by HPLC. Methods: The mobile phase was eluted with a gradient of 0.4% phosphoric acid in methanol and on an Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm) using RP-HPLC at a flow rate of 0.8 mL · min- 30 ° C. Results: The linear ranges of gallic acid, protocatechuic acid, corilagin and ellagic acid were 0.059-2.360 gL-1 (r = 0.999 6), 0.017-0.667 g g-1 (r = 0.999 9), 0.351-14.04 gL-1 (r = 0.999 9), 0.151-6.040 gL-1 (r = 0.999 8). The average recoveries (n = 3) were 99.45 (RSD 1.5%), 98.65% (RSD 1.7%), 100.3% (RSD 2.0%) and 98.90% (RSD 1.2%), respectively. Conclusion: The determination of content of this method is rapid, accurate, reliable, reproducible, and can provide the basis for the quality control of medicinal materials.