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目的做为中国急性弛缓性麻痹(AFP)病例监测系统和中国维持无脊髓灰质炎(脊灰)状态的一部分,对日喀则地区2005年健康儿童肠道病毒(EV)的携带情况进行监测分析,为西藏自治区维持无脊灰提供实验室依据。方法2005年3月在日喀则地区3个县的0～5岁健康儿童中共采集粪便标本70份,进行EV分离和血清型鉴定。对分离到的脊灰病毒(PV)同时使用逆转录-聚合酶链反应和酶联免疫吸附试验进行型内鉴定,对分离到的所有非脊灰肠道病毒(NPEV)进行VP1区核苷酸序列测定以鉴定血清型。结果从70份粪便标本中共分离到16株PV,阳性率为22.86%。型内鉴定及VP1区核苷酸序列测定和分析结果显示,2005年日喀则地区无脊灰野病毒和疫苗衍生脊灰病毒的存在;共分离到9株NPEV,阳性率为12.86%。结论日喀则地区2005年继续保持无脊灰状态。EV监测为后续研究提供宝贵的资料,也为EV监测逐渐整合到AFP病例监测系统中做了有益的尝试。
Objective To monitor and analyze the carrying status of enterovirus (EV) in healthy children in Shigatse region in 2005 as part of the surveillance system of acute flaccid paralysis (AFP) cases and the maintenance of non-polio (polio) status in China. Tibet Autonomous Region to maintain polio-free laboratory basis. Methods In March 2005, 70 stool samples were collected from healthy children aged 0 ~ 5 years in 3 counties of Shigatse region for EV isolation and serological identification. The isolated poliovirus (PV) was genotyped by reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA), and all the isolated non-polio enterovirus (NPEV) Sequencing assays to identify serotypes. Results A total of 16 PVs were isolated from 70 stool specimens. The positive rate was 22.86%. The result of in-type identification and nucleotide sequence analysis of VP1 showed that there was no poliovirus and vaccine-derived poliovirus in Xigaze region in 2005. Nine NPEV strains were isolated and the positive rate was 12.86%. Conclusion The Xigaze region continued to be pollinated in 2005. EV monitoring provides valuable information for the follow-up study, but also makes a beneficial attempt for the gradual integration of EV monitoring into AFP case monitoring system.