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目的： 研究维生素Ｅ（ＶＥ）、硒（Ｓｅ）及二者联合应用对四氯化碳（ＣＣｌ４）作用下肝细胞、储脂（Ｉｔｏ）细胞、细胞外基质（ＥＣＭ）产生的抑制作用。方法： 采用大鼠肝脏原位灌流、分离培养肝细胞和Ｉｔｏ 细胞。结果： ＶＥ、Ｓｅ 能明显抑制ＣＣｌ４ 作用下大鼠肝细胞、Ｉｔｏ 细胞３Ｈ－脯氨酸（３Ｈ－Ｐｒｏ）掺入、Ⅲ型前胶原（ＰＣⅢ）分泌，还可使Ｉｔｏ 细胞透明质酸（ＨＡ）、板层素（ＬＮ）、纤维连接素（ＦＮ）分泌减少。ＶＥ加Ｓｅ在抑制肝细胞、Ｉｔｏ 细胞３Ｈ－Ｐｒｏ 掺入、降低丙二醛（ＭＤＡ）释出，提高谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性，降低Ｉｔｏ 细胞ＨＡ、ＦＮ分泌诸方面优于ＶＥ或Ｓｅ 的单独作用，并可使Ｓｅ 的用量节省一半。结论： ＶＥ和Ｓｅ具有抑制大鼠肝细胞、Ｉｔｏ 细胞ＥＣＭ的作用；二者的联合应用效果更好，可望成为一种抗肝纤维化的新方法。
Objective: To study the inhibitory effect of Vitamin E (VE), selenium (Se) and their combination on hepatocytes, Ito cells and extracellular matrix (ECM) under the action of carbon tetrachloride (CCl4). Methods: Rat liver was perfused in situ and hepatocytes and Ito cells were isolated and cultured. Results: VE and Se significantly inhibited the incorporation of 3H-Pro into Ito cells and the secretion of type III procollagen (PCⅢ) in CCl4-treated rat hepatocytes, ), Laminin (LN), fibronectin (FN) secretion decreased. VE plus Se inhibits the incorporation of 3H-Pro in hepatocytes and Ito cells, decreases the release of malondialdehyde (MDA), increases the activity of glutathione peroxidase (GSH-Px), and decreases the secretion of HA and FN in Ito cells It is better than VE or Se alone, and can save Se dosage by half. Conclusion: VE and Se can inhibit the ECM of rat hepatocytes and Ito cells. The combined effect of the two is better and is expected to become a new anti-fibrosis method.