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目的对北京市夏季市售水产品污染与感染病例中副溶血性弧菌血清型和毒力基因型进行比较研究,为评估食品安全风险监测的目的与意义提供思路,为北京市水产品副溶血性弧菌污染与临床感染病例的关联性研究提供技术支持。方法对采集的水产品样品和哨点医院腹泻患者粪便样本进行副溶血性弧菌的分离鉴定,采用血清玻片凝集法对分离出的副溶血性弧菌进行血清分型,PCR方法检测菌株的tlh、tdh、trh基因。结果 2014年7~9月共采集水产品样品164份,检出副溶血性弧菌80份,总污染率为48.78%;其中淡水产品污染率为38.78%(19/49),平均菌量浓度为66.63 MPN/g;海水产品污染率为53.04%(61/115),平均菌量浓度为38.14 MPN/g。80株副溶血性弧菌分属于9个血清群,其中O2群28株,占35.00%,O1群11株,占13.75%,O5群10株,占12.50%。80株菌tlh基因均为阳性,只有1株菌携带tdh毒力基因,所有菌株trh毒力基因均为阴性。哨点医院腹泻病人粪便样本中分离鉴定副溶血性弧菌21株,血清型O3∶K6占61.90%(13/21),O4:K8占28.57%(6/21);毒力基因型tdh(+)/trh(-)占95.24%(20/21),tdh(-)/trh(-)占4.76%(1/21)。结论来源于食品样品的副溶血性弧菌绝大部分不具备致病性,而导致消费者腹泻的副溶血性弧菌绝大部分携带致病性毒力基因,表明目前的食品安全风险监测结果不能作为评估副溶血弧菌导致的食源性疾病暴发和散发的依据。
Objective To compare the serum and virulence genotypes of Vibrio parahaemolyticus in the aquatic products contaminated and infected in summer in Beijing, and to provide ideas for evaluating the purpose and significance of food safety risk monitoring. Vibrio parahaemolyticus and clinical infection cases to provide technical support for the study. Methods Isolation and identification of Vibrio parahaemolyticus from diarrhea patients in samples collected from aquatic products and sentinel hospitals, and serum Vibrio parahaemolyticus was detected by serum slide agglutination method. PCR was used to detect the isolates of Vibrio parahaemolyticus tlh, tdh, trh genes. Results A total of 164 samples of aquatic products were collected from July to September 2014. 80 strains of Vibrio parahaemolyticus were detected with the total contamination rate of 48.78%. The freshwater product contamination rate was 38.78% (19/49), the average bacterial concentration Which was 66.63 MPN / g. The pollution rate of seawater products was 53.04% (61/115), and the average bacterial concentration was 38.14 MPN / g. 80 Vibrio parahaemolyticus belonged to 9 serogroups, of which 28 were O2 group, accounting for 35.00%, 11 were O1 group (13.75%) and 10 were O5 group (12.50%). 80 strains of bacteria tlh gene were positive, only one strain carrying tdh virulence genes, all strains of trh virulence genes were negative. 21 strains of Vibrio parahaemolyticus were isolated and identified in the stool samples of sentinel hospital with diarrhea. The serotype O3: K6 accounted for 61.90% (13/21), O4: K8 accounted for 28.57% (6/21), the virulence genotype tdh +) / trh (-) accounted for 95.24% (20/21) and tdh (-) / trh (-) accounted for 4.76% (1/21). Conclusion The majority of Vibrio parahaemolyticus originated from food samples do not have pathogenicity, while the majority of Vibrio parahaemolyticus causing diarrhea in consumers carry pathogenic virulence genes, indicating that the current food safety risk monitoring results It can not be used as a basis for assessing the outbreak and distribution of food borne diseases caused by Vibrio parahaemolyticus.