观察骨痛仙胶囊对骨折愈合过程中Ⅰ、Ⅱ型胶原mRNA的转录水平的影响,分析其促进骨折愈合的分子机理。方法健康成年家兔45只,做双侧桡骨骨折模型,随机分成治疗组、阳性对照组、模型组,分别给予骨痛仙胶囊、骨折挫伤散和生理盐水灌胃给药,术后1周、2周、4周后处死家兔取材,用原位杂交法测定骨Ⅰ、Ⅱ型胶原表达,与模型组、阳性对照组比较进行病理学观察。结果各时间段治疗组Ⅰ型胶原mRNA的转录水平均增强,明显高于阳性对照组和模型组(P<0.05)。与对照组和模型组相比,给药组Ⅱ型胶原mRNA的转录水平第1周明显提高,第2、4周Ⅱ型胶原明显衰落,与模型组、阳性对照组相比有统计学意义(P<0.05)。结论骨痛仙胶囊与阳性对照组骨折挫伤散均能调节骨折愈合中的相关胶原基因的表达,促进骨折愈合;两组之间相比,骨痛仙胶囊调节骨折愈合中的Ⅰ、Ⅱ型胶原基因表达明显优于阳性对照组。 Observe the effect of Gutongxian Capsule on the transcription of type I and type II collagen mRNA during fracture healing, and analyze its molecular mechanism to promote fracture healing. Methods Forty-five healthy rabbits were used as the bilateral tibial fracture models and randomly divided into treatment group, positive control group, and model group. They were given Guanguxian capsule, fracture contusion powder, and saline, respectively. One week after surgery. After 2 weeks and 4 weeks, rabbits were sacrificed. The expression of type I and type II collagen was measured by in situ hybridization. The pathological observation was performed comparing with the model group and the positive control group. Results The transcription level of type I collagen mRNA was significantly increased in the treatment group at each time point, which was significantly higher than that of the positive control group and the model group (P<0.05). Compared with the control group and the model group, the transcription level of type II collagen mRNA in the administration group was significantly increased at the first week, and type II collagen was significantly declined at the second and fourth week, which was statistically significant compared with the model group and the positive control group ( P<0.05). Conclusion Both Gutongxian capsule and positive control group can reduce the expression of related collagen gene in fracture healing and promote fracture healing. Compared with the two groups, Guantongxian Capsule can regulate Type I and Type II collagen in fracture healing. Gene expression was significantly better than that of the positive control group.