论文部分内容阅读
目的探讨左向右分流大鼠肺血管超微结构的动态变化及与内源性硫化氢(H2S)的关系。方法经下腔静脉-腹主动脉穿刺术建立左向右分流动物模型,分别在分流术后1、3d,1、4、8周各实验时间点处死大鼠,制作肺组织电镜标本,并用敏感硫电极法测量大鼠血浆H2S含量。结果肺血管超微结构动态变化为进行性加重,首先为内皮细胞改变,然后为平滑肌细胞,最后出现细胞外基质异常堆积。分流组大鼠血浆H2S含量在分流术后4周与对照组比较明显增高。结论左向右分流大鼠肺血管超微结构动态变化中伴随内源性H2S变化,提示内源性H2S可能在肺血管结构改变中发挥调节作用。
Objective To investigate the dynamic changes of pulmonary vascular ultrastructure and its relationship with endogenous hydrogen sulfide (H2S) in left-to-right shunt rats. Methods The left-right shunt animal model was established by inferior vena cava-abdominal aorta puncture. Rats were sacrificed at 1, 3, 1, 4, and 8 weeks after shunt, respectively. Electron microscopy (TEM) Measurement of plasma H2S level in rat plasma by sulfur electrode method. Results The dynamic changes of pulmonary vascular ultrastructure were progressive aggravating. First, endothelial cells changed, then smooth muscle cells, finally the abnormal accumulation of extracellular matrix appeared. The plasma level of H2S in shunt group was significantly increased at 4 weeks after shunt compared with the control group. Conclusion The dynamic changes of pulmonary vascular ultrastructure in rats with left-to-right shunt are accompanied by changes of endogenous H2S, suggesting that endogenous H2S may play a regulatory role in pulmonary vascular structural changes.