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目的 探讨低血糖状态下血浆和组织中谷胱甘肽含量的变化以及还原型谷胱甘肽 (GSH)对血清酶活性的抑制作用。方法 静脉注射胰岛素诱发低血糖 ,并持续 60min ,然后输注葡萄糖解除低血糖。结果发现诱发低血糖后 6h ,血浆GSH、氧化型谷胱甘肽 (GSSG)、谷胱甘肽总量 (TGSH)及GSSG/TGSH比值均明显升高 ,肝脏GSH和TGSH明显减少 ,心肌和骨骼肌GSSG和GSSG/TGSH比值明显增高 ,同时伴有血清酶 (ALT、AST、LDH、CK)活性升高。预先注射GSH后诱发低血糖组 ,血浆GSSG和GSSG/TGSH比值明显下降 ,并抑制了血清酶的活性。结论 低血糖所致的血清酶活性的升高与体内谷胱甘肽氧化还原状态的改变有关 ,补充抗氧化剂GSH可以预防低血糖所致的肌肉损伤
Objective To investigate the changes of glutathione content in plasma and tissue under hypoglycemic condition and the inhibitory effect of reduced glutathione (GSH) on serum enzymatic activity. Methods Insulin-induced hypoglycemia was given intravenously for 60 minutes, then glucose was infused to relieve hypoglycemia. The results showed that at 6h after induction of hypoglycemia, the ratios of plasma GSH, GSH, GSH and GSSG / TGSH were significantly increased, the hepatic GSH and TGSH were significantly decreased, while the myocardial and skeletal Muscle GSSG and GSSG / TGSH ratio was significantly increased, accompanied by elevated serum enzymes (ALT, AST, LDH, CK). Hypoglycemia group was induced after pre-injection of GSH, the ratio of plasma GSSG and GSSG / TGSH was significantly decreased, and the activity of serum enzyme was inhibited. Conclusions The increase of serum enzyme activity induced by hypoglycemia is related to the change of glutathione redox status in vivo. Supplementation of antioxidant GSH can prevent muscle damage caused by hypoglycemia