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目的分析脂多糖致早期新生大鼠脑白质损伤中Nrf2及GSTP1蛋白的表达水平。方法将64只新生SD大鼠随机分为生理盐水对照组及脂多糖组,脂多糖组新生大鼠于出生后第2~6天连续腹腔注射脂多糖0.6 mg·kg~(-1)·d~(-1),对照组注射等量的生理盐水。分别于腹腔注射后12 h、24 h、72 h、5 d 4个时间点每组各处死8只大鼠,取脑白质,HE染色后光镜下观察脑白质区病理变化;采用免疫组织化学方法及Western-印迹方法检测脑白质区Nrf2及GSTP1蛋白的表达变化。结果脂多糖组的Nrf2及GSTP1的阳性细胞数及其蛋白表达均在12 h开始增加,24 h时达到高峰,而后逐渐减少,不同时间点间两两比较,差异均有统计学意义(P<0.05);与脂多糖组比较,生理盐水对照组各时间点脑白质可见Nrf2及GSTP1的少量表达,各时间点间的差异无统计学意义(P>0.05)。结论 Nrf2及GSTP1可能参与早期新生大鼠感染后引起脑白质损伤的病理过程,而高表达的Nrf2及GSTP1蛋白可能对其有保护作用。
Objective To analyze the expression of Nrf2 and GSTP1 in white matter damage induced by lipopolysaccharide in early neonatal rats. Methods Sixty-four newborn Sprague-Dawley rats were randomly divided into normal saline control group and lipopolysaccharide group. LPS rats were injected intraperitoneally with LPS (0.6 mg · kg -1 · d) on the 2nd to 6th day after birth ~ (-1), the control group was injected with the same amount of saline. Eight rats were sacrificed at 4, 12, 24, 72, and 5 days after intraperitoneal injection respectively. Pathological changes of white matter were observed under light microscope with HE staining. Immunohistochemistry Methods Western blotting was used to detect the expression of Nrf2 and GSTP1 protein in white matter of brain. Results The number of positive cells and protein expression of Nrf2 and GSTP1 in the lipopolysaccharide group began to increase at 12 h, peaked at 24 h, and then decreased gradually. The difference was statistically significant between different time points (P < 0.05). Compared with the lipopolysaccharide group, the expression of Nrf2 and GSTP1 in the white matter of normal saline control group at each time point was small, with no significant difference at each time point (P> 0.05). Conclusion Nrf2 and GSTP1 may be involved in the pathological process of white matter damage induced by early neonatal infection, while the high expression of Nrf2 and GSTP1 proteins may be protective effect.