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为明确内蒙古马铃薯黑痣病菌融合群的组成及其致病力,从内蒙古13个县(旗)分离得到109株黑痣病菌,采用载玻片配对法和土壤接种法分别测定其融合群及致病力。结果表明,109株黑痣病菌中共存在8个融合群及亚群,分别为AG1-IB、AG2-1、AG3、AG4-HG-Ⅱ、AG4-HG-Ⅲ、AG5、AG9及AG-A。其中84株菌株为AG3,占77.06%;AG4-HG-Ⅱ和AG5分别为8株和6株,占7.34%和5.50%;其余5个融合群各不超过5株,所占比例均在5.00%以下。在84株AG3融合群中,从薯块分离得到52株,茎表分离得到32株;其它7个融合群共25株菌株均分离自茎表。除AG-A无致病能力外,其它融合群均可致病,AG3致病力最强,AG4和AG5次之,AG2-1、AG9和AG1-IB最弱。表明内蒙古马铃薯黑痣病菌具有丰富的多样性,但其优势融合群仍为AG3。
In order to clarify the composition and virulence of potato herpet mosaic virus in Inner Mongolia, 109 strains of M. albicans were isolated from 13 counties in Inner Mongolia, and their fusion groups were determined by the method of slide pairing and soil inoculation Disease. The results showed that there were 8 fusion groups and subgroups among 109 strains of V. albicans, which were AG1-IB, AG2-1, AG3, AG4-HG-Ⅱ, AG4-HG-Ⅲ, AG5, AG9 and AG- Among them, 84 strains were AG3, accounting for 77.06%; AG4-HG-Ⅱ and AG5 were 8 and 6, accounting for 7.34% and 5.50% respectively; the remaining 5 were no more than 5, accounting for 5.00 %the following. Among 84 AG3 fusion groups, 52 strains were isolated from potato tuber and 32 strains were isolated from stems. The other 7 strains were isolated from the stems of 25 strains. In addition to the non-pathogenic ability of AG-A, other fusion groups were pathogenic, AG3 virulence was the strongest, followed by AG4 and AG5, AG2-1, AG9 and AG1-IB the weakest. The results showed that the diversity of potato pathogens in Inner Mongolia was rich, but its dominant fusion group was still AG3.