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目的 探讨肺循环血液流变学在急性呼吸窘迫综合征 (ARDS)发病中的变化及在ARDS肺循环障碍中的可能作用。方法 通过尾静脉注射油酸 (OA)制备大鼠ARDS模型 ,动态检测动物模型 (0 5h ,2h ,6h ,12h ,2 4h ,72h)肺动脉血、颈动脉血和静脉血在不同切变率下的全血粘度 ,同时进行全血细胞分析。结果 OA型ARDS大鼠的肺、体循环血液在高切变率 (2 0 0s-1,30s-1)下的粘度均无明显变化。在低切变率 (5s-1)时 ,6h～ 2 4hARDS大鼠肺循环血全血粘度均显著高于对照组 ;切变率为 1s-1时 ,2hARDS大鼠肺循环血全血粘度即显著升高 ,6h和 12h不仅明显高于对照组 ,也高于自身静脉血 ;体循环血粘度变化不明显。 2h～ 2 4hARDS大鼠肺、体循环血中红细胞和血红蛋白浓度均有增加 ,血小板数则呈减少趋势。结论 OA型ARDS大鼠肺循环血液在低切变率下的粘度增加 ,可通过增加血流阻力和减慢流速 ,恶化机体的缺氧状态
Objective To investigate the changes of pulmonary hemorheology in the pathogenesis of acute respiratory distress syndrome (ARDS) and the possible role in ARDS pulmonary circulation disorders. Methods Rat ARDS model was established by injecting oleic acid (OA) via the tail vein. The dynamic changes of pulmonary artery, carotid artery and venous blood were detected dynamically at different shear rates in animal models (0 5h, 2h, 6h, 12h, 24h, 72h) Of the whole blood viscosity, while the whole blood cell analysis. Results There was no significant change in the viscosity of lung and systemic blood of OA type ARDS rats at high shear rate (200s-1, 30s-1). At low shear rate (5s-1), whole blood viscosity of pulmonary circulation in 6h ~ 24hARDS rats was significantly higher than that in control group. Viscosity of pulmonary blood in 2hARDS rats was significantly increased at 1s-1 shear rate High, 6h and 12h not only significantly higher than the control group, but also higher than their venous blood; changes in systemic blood viscosity was not obvious. 2h ~ 2 4hARDS rat lung, systemic blood red blood cells and hemoglobin concentrations have increased, the number of platelets showed a decreasing trend. Conclusion The viscosity of pulmonary circulation blood of OA type ARDS rats increases at low shear rate, which can worsen the hypoxia state by increasing the blood flow resistance and slowing the flow rate