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目的研究温室土壤中有机提取物(extractable organic matter,EOM)对小鼠肝肾功能的影响。方法于2010年5月采集并提取某蔬菜大棚土壤中的EOM。将40只健康10周龄清洁级昆明雄性小鼠随机分为4组,分别为对照(DMSO-植物油体积比为6︰4)组和低剂量(0.5 g/ml)、中剂量(1.5 g/ml)和高剂量(3 g/ml)EOM染毒组,每组10只。采用灌胃方式连续染毒4周。检测肝、肾组织匀浆和血清中的超氧化物歧化酶(SOD)活力与丙二醛(MDA)含量以及肝肾功能相关生化指标[丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)的活力和总蛋白(TP)、白蛋白(ALB)、球蛋白(GLO)、总胆固醇(TCH)、甘油三酯(TG)、肌酐(cr)、尿素氮(BUN)、尿酸(UA)、血糖(GLU)含量的测定,并计算ALT/AST、白蛋白/球蛋白(A/G)值。结果与对照组比较,1.5、3.0 g/ml EOM染毒组小鼠肝、肾匀浆中SOD活力均下降,MDA含量均升高;各剂量EOM染毒组小鼠血清中仅MDA含量明显升高,差异有统计学意义(P<0.05或P<0.01)。且随着EOM染毒剂量的升高,小鼠肝、肾匀浆中SOD活力呈剂量依赖性下降(P<0.05),肝、肾匀浆和血清中MDA含量呈剂量依赖性上升(P<0.05)。与对照组比较,各剂量EOM染毒组小鼠血清中ALT活力及1.5、3.0 g/ml EOM染毒组小鼠血清中AST活力和cr、BUN含量以及1.5 g/ml EOM染毒组小鼠血清中ALP活力均明显升高,0.5、1.5 g/ml EOM染毒组小鼠血清中TG含量均明显下降,差异有统计学意义(P<0.05或P<0.01);此外,0.5 g/ml EOM染毒组小鼠血清中GLO含量明显升高,A/G值明显下降,差异有统计学意义(P<0.05);1.5、3.0 g/ml EOM染毒组小鼠血清中UA和3.0 g/mlEOM染毒组小鼠血清中GLU含量明显下降,差异有统计学意义(P<0.05);而各剂量EOM染毒组小鼠血清中TP、ALB、TCH含量及ALT/AST值均无显著变化。随着EOM染毒剂量的升高,小鼠血清中AST活力和BUN含量均呈剂量依赖性升高(P<0.05),GLU含量呈剂量依赖性下降(P<0.05);其他指标均未发现明显相关关系。结论温室土壤中EOM可诱导小鼠肝、肾组织产生脂质过氧化损伤作用。
Objective To study the effects of extractable organic matter (EOM) on liver and kidney function in mice. Methods In May 2010, the EOM in a vegetable greenhouse was collected and extracted. Forty healthy Kunming male mice, aged 10 weeks, were randomly divided into 4 groups: control (DMSO - vegetable oil volume ratio of 6: 4) group and low dose (0.5 g / ml) ml) and high dose (3 g / ml) EOM group, 10 rats in each group. Gavage continuous exposure for 4 weeks. The activities of superoxide dismutase (SOD) and malondialdehyde (MDA) in the homogenate and serum of liver and kidney were detected, and the biochemical indexes related to liver and kidney function [alanine aminotransferase (ALT) and aspartate (AST), alkaline phosphatase (ALP) activity and total protein (TP), albumin (ALB), globin (GLO), total cholesterol (TCH), triglyceride (TG), creatinine Cr, BUN, UA and GLU, and calculate ALT / AST and albumin / globulin (A / G) values. Results Compared with the control group, the activity of SOD in the liver and kidney homogenates of mice in the EOM group was decreased and the content of MDA was increased in the EOM group; only the content of MDA in the serum of the EOM group was significantly increased High, the difference was statistically significant (P <0.05 or P <0.01). And with the increase of EOM dose, the activity of SOD in liver and kidney homogenate decreased in a dose-dependent manner (P <0.05), and the content of MDA in liver and kidney homogenate and serum increased in a dose-dependent manner (P < 0.05). Compared with the control group, ALT activity in serum of mice in each dose of EOM and the activity of AST and cr, BUN in serum of 1.5, 3.0 g / ml EOM group and 1.5 g / ml EOM group Serum ALP activity were significantly increased, 0.5,1.5 g / ml EOM exposure group mice serum TG levels were significantly decreased, the difference was statistically significant (P <0.05 or P <0.01); addition, 0.5 g / ml Serum GLO levels in EOM-treated mice significantly increased, and A / G decreased significantly (P <0.05). Serum levels of UA and 3.0 g (P <0.05). However, the levels of TP, ALB, TCH and ALT / AST in serum of EOM-treated mice were not significantly different Variety. With the increase of EOM dose, AST and BUN levels in serum increased in a dose-dependent manner (P <0.05), while GLU level decreased in a dose-dependent manner (P <0.05). No other indexes were found Clearly related. Conclusion EOM in the greenhouse soil can induce lipid peroxidation injury in the liver and kidney of mice.