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目的探讨建立同时测定跌打丸中芍药苷、川续断皂苷Ⅵ和丹皮酚含量的方法。方法采用Agilent ZORBAX C_(18)色谱柱(4.6 mm×250 mm,5μm);流速为0.8 m L·min~(-1),以乙腈(A)-0.1%磷酸水溶液(B)为流动相,进行梯度洗脱;检测波长为230,212,274 nm。结果芍药苷进样量在0.514 8~1.544 5μg与峰面积线性关系良好(r=0.999 7),平均回收率为97.01%(RSD=0.94%,n=6)。川续断皂苷Ⅵ在3.023 8~9.071 4μg线性关系良好(r=0.999 2),平均回收率为98.44%(RSD=0.26%,n=6)。丹皮酚在0.101 2~0.303 6μg线性关系良好(r=0.999 8),平均回收率为97.84%(RSD=0.42%,n=6)。结论建立的方法简便可行,重复性好,灵敏度高,可有效控制跌打丸的质量。
Objective To establish a method for simultaneous determination of paeoniflorin, chuanzuo saponin Ⅵ and paeonol in oridiwan. Methods The mobile phase consisted of acetonitrile (A) - 0.1% phosphoric acid solution (B) with Agilent ZORBAX C 18 column (4.6 mm × 250 mm, 5 μm) at a flow rate of 0.8 m L · min -1. Gradient elution; detection wavelength of 230,212,274 nm. Results Paeoniflorin had a good linearity (r = 0.999 7) with an average recovery of 97.01% (RSD = 0.94%, n = 6) in the range of 0.514 8-1.544 5 μg. The linear relationship was good between 3.023 8 and 9.071 4μg (r = 0.999 2), and the average recovery was 98.44% (RSD = 0.26%, n = 6). Paeonol showed a good linearity (r = 0.999 8) with an average recovery of 97.84% (RSD = 0.42%, n = 6) at 0.101 2-0.303 6 μg. Conclusion The established method is simple and feasible, good repeatability and high sensitivity, which can effectively control the quality of pills.