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目的探讨血管性血友病因子裂解蛋白酶(ADAMTS13)、凝血酶敏感蛋白1(TSP1)及C1423T基因多态性在老年急性脑梗死患者中的意义。方法选取62例老年急性脑梗塞患者和56例与之匹配的健康对照者,用vWF:Ag和TSP1 ELISA试剂盒对两组血浆中的vWF:Ag和TSP1含量进行检测,同时残余胶原结合实验(R-CBA)检测两组血浆中的ADAMTS13活性水平,聚合酶链反应(PCR)扩增并限制性酶切扩增产物测定ADAMTS13基因C1423T多态性。结果急性脑梗死患者血浆中vWF:Ag和TSP1含量均高于正常组(P<0.05),ADAMTS13活性水平低于正常组(P<0.05);62例急性脑梗死患者中3例出现C1423T基因突变(4.84%),高于正常组(0%)。结论 ADAMTS13活性降低可能参与脑梗死的发生发展,血浆中的TSP1水平在一定程度上可能促进ADAMTS13对血管性血友病因子(vWF)的裂解;中国汉族人群中基因C1423T突变可能降低ADAMTS13的活性水平,从而参与脑梗死的发生。
Objective To investigate the significance of polymorphisms of ADAMTS13, TSP1 and C1423T in elderly patients with acute cerebral infarction. Methods Sixty-two elderly patients with acute cerebral infarction and 56 matched controls were enrolled in this study. The vWF: Ag and TSP1 levels in plasma were detected by vWF: Ag and TSP1 ELISA kit. The residual collagen binding assay R-CBA) was used to detect the level of ADAMTS13 activity in plasma. The C1423T polymorphism of ADAMTS13 gene was detected by polymerase chain reaction (PCR) amplification and restriction endonuclease amplification. Results The levels of vWF: Ag and TSP1 in plasma of patients with acute cerebral infarction were significantly higher than those in normal group (P <0.05), and the levels of ADAMTS13 in patients with acute cerebral infarction were lower than those in normal group (P <0.05). C1423T gene mutation was found in 3 of 62 acute cerebral infarction patients (4.84%), higher than the normal group (0%). Conclusion The decreased activity of ADAMTS13 may be involved in the development of cerebral infarction. The level of TSP1 in plasma may promote the cleavage of vWF by ADAMTS13. The mutation of C1423T in Chinese Han population may reduce the activity of ADAMTS13 , Which involved in the occurrence of cerebral infarction.