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目的:观察尿感方对尿道致病性大肠杆菌(UEC)在人尿道上皮细胞黏附效应的影响。方法:体外实验以营养肉汤组作空白对照,观察经三金片、蔓越莓、不同浓度的尿感方等药物处理后人尿道上皮细胞上UEC黏附数量的变化。体内实验比较经正常尿液及口服尿感方后4 h尿液、次日晨尿处理后的人尿道上皮细胞上UEC黏附数量的变化。结果:①体外实验:经不同药物处理后的各用药组其UEC黏附于尿道上皮细胞上的细菌数较未经药物处理的营养肉汤组明显减少(P<0.01);三金片、蔓越莓组与同等浓度的尿感方组比较,其黏附在尿道上皮细胞上的UEC细菌数无明显差异(P>0.05);三种不同浓度的尿感方组比较,尿感方浓度越高,抗黏附效果越好。②体内实验:经口服尿感方后4 h尿及次日晨尿处理过的UEC黏附于尿道上皮细胞上的数目较经服药前的正常尿液处理的UEC黏附数目明显减少(P<0.05,P<0.01)。结论:尿感方具有良好的体外、体内抗UEC黏附的作用,且药物浓度越高,抗黏附效果越好。尿感方阻止UEC在人尿道上皮细胞的黏附可能是其治疗尿路感染的作用机制之一。
Objective: To observe the effect of urinary flu prescription on the adhesion of urethral pathogenic Escherichia coli (UEC) to human urothelial cells. Methods: In vitro experiments, a blank control group was treated with nutrient broth to observe the changes of the number of UEC adhered to human urethra epithelial cells treated with three gold tablets, cranberry, different concentrations of urinary flu prescription and so on. In vivo experiments compared the normal urine and oral urine sensory side after 4 h urine, the next day morning urine URE epithelial cells after the number of changes in adhesion. Results: In vitro experiments: The number of bacteria adhered to urethral epithelial cells by UEC in each drug-treated group decreased significantly compared with those in untreated nutrient broth group (P <0.01) There was no significant difference in the number of UEC bacteria adhering to the urethral epithelial cells between the raspberry group and the urine of the same concentration (P> 0.05). Compared with the other three groups, the urinary tract concentration was higher, Anti-adhesion effect is better. ②In vivo, the number of UEC adhering to urethral epithelial cells treated with urinary 4h urine and the next morning urine after oral administration of urinary flu prescription was significantly decreased compared with that of normal urinary UEC treated by medication (P <0.05, P <0.01). Conclusion: Urine flu prescription has a good anti-UEC adhesion effect in vitro and in vivo, and the higher the drug concentration, the better anti-adhesion effect. Urinary flu prescription to prevent UEC adhesion in human urothelial cells may be one of its mechanisms of treatment of urinary tract infection.