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我院于1979年开始根据国内外文献所提供的方法,改用超过滤法,先后研制成牛、猪胸腺素组分V(THF_5),经冻干制成粉针剂。几年来,经动物实验及临床观察,发现对抗癌、抗放、抗衰老等有一定临床作用。现将研究结果报告如下:一、THF_5生产工艺及质量检测我院根据Goldstein Hooper及刘士廉等的方法,将牛、猪胸腺经抽提、盐析、脱盐、超滤、分装制成冻干THF_5粉针剂,经各项试验证实有活性后方供临床使用。二、THF_5动物试验情况1.猪、牛THF_5生物特性对比为观察猪、牛胸腺素特性,经分析发现两者蛋白质含量基本一致,对活性玫瑰花结的影响,猪THF_5活性略低于牛THF_5。两者对骨髓中T细胞分化的影响无甚差别,两种制剂经SDS电泳与聚焦电泳,发现它们分子量基本一致。2.THF_5对造血系统的作用(1)THF_5对小鼠粒系造血祖细胞的增殖作用选择24～28克雄性瑞士纯种小鼠,每组6只,1～5组,每只小鼠分别用THF_5 5μg连续腹腔注射5、10、15、20、25天,第6组不处理作为对照组,两组选择条件相同。
Our hospital began in 1979 according to the methods provided by domestic and foreign literature, the use of ultrafiltration method, has developed into cattle, pig thymosin component V (THF_5), made by freeze-dried powder injection. In recent years, animal experiments and clinical observations, found that anti-cancer, anti-release, anti-aging and some have a clinical role. The research results are reported as follows: First, the production process and quality testing of THF_5 According to the methods of Goldstein Hooper and Liu Shilian et al., The cow, pig thymus were extracted, salted out, desalted, Powder injection, confirmed by various tests for the active rear for clinical use. Second, the THF_5 animal test 1. Pig, bovine THF_5 biological characteristics comparison To observe the characteristics of pigs, bovine thymosin, the analysis found that the two proteins are basically the same, the activity of the rosettes, the pig activity was slightly lower THF_5 . There was no difference between the two in the differentiation of T cells in bone marrow. The two preparations were found to be basically the same in molecular weight by SDS electrophoresis and focusing electrophoresis. The effect of THF_5 on the hematopoietic system (1) Proliferation of mouse granulocyte-derived hematopoietic progenitor cells by THF_5Selected 24-28 g male Swiss purebred mice, 6 mice in each group, 1 to 5 mice each The mice were injected intraperitoneally with THF_5 5μg for 5, 10, 15, 20, and 25 days. The sixth group was not treated as the control group. The two groups had the same selection conditions.