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Objective To strengthen the surveillance and control of nosocomial infection in neonatal ICU. Methods To be seriously considered by leaders, to carry out rules and systems, to strengthen education and enhance consciousness of infections, to carry out targeted surveillance on basis of routine surveillance. Results The consciousness of infections has been enhanced. Passive carrying out of sterilization and isolation has tued active carrying out. The air quality in NICU has been improved. Ventilator - related infections have been decreased. Conclusion To strengthen surveillance and control of nosocomial infection in neonatal ICU and to find out and solve the weak link in infection control is the key to control nosocomial infection, to insure medical safety, and to improve medical quality.