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为了更好的利用乌克兰小麦品种资源,并了解引进品种的品质,采用SDS-PAGE和A-PAGE技术,对从乌克兰引进小麦材料的高分子量麦谷蛋白亚基(HMW-GS)和醇溶蛋白亚基的组成进行分析。结果表明,在16个普通小麦品种中,由Glu-1位点控制的高分子量亚基组合类型共有7种,最常见的是(1,7+8,5+10)占37.5%,其次是(1,2+12,6+8)和(1,7+9,5+10),各占18.75%,其中Glu-A1位点有3种等位变异,以1亚基为主(75%);Glu-B1位点有3种等位变异,以7+8为主(43.75%);Glu-D1位点有3种等位变异,以5+10为主(68.75%)。醇溶蛋白方面,从供试材料的6个位点中,共鉴定了33个不同的醇溶蛋白等位基因,41条迁移率不同的醇溶蛋白带纹,其中Gli-A1,Gli-B1和Gli-D1分别有6,5,5个等位基因;Gli-A2,Gli-B2和Gli-D2各有6,5,6个等位基因,优质亚基Gli-B1b出现频率较高(43.75%),这些材料有可能会成为比较有价值的品质改良中间材料。
In order to make better use of Ukrainian wheat germplasm resources and to understand the quality of imported varieties, SDS-PAGE and A-PAGE were used to analyze the HMW-GS and prolamine subunits The composition of the base is analyzed. The results showed that in 16 common wheat cultivars, there were 7 types of high molecular weight subunit combinations controlled by Glu-1 locus, the most common ones were (1, 7 + 8, 5 + 10) accounted for 37.5% (1, 2 + 12, 6 + 8) and (1,7 + 9, 5 + 10), accounting for 18.75% respectively. There were 3 alleles at Glu-A1 locus, %). There were three kinds of allelic variation at Glu-B1 loci, mainly 7 +8 (43.75%). There were three allelic variations at Glu-D1 loci, which were predominantly 5 + 10 (68.75%). In terms of gliadin, 33 different gliadin alleles and 41 gliadin bands with different mobility were identified from 6 loci of the tested materials, of which Gli-A1, Gli-B1 Gli-A2, Gli-B2 and Gli-D2 each had 6, 5, and 6 alleles, and the high-quality subunit Gli-B1b appeared more frequently 43.75%), these materials are likely to become more valuable quality improvement intermediate materials.