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应用原代培养人小肠上皮细胞(IEC),观察缺氧复氧(A/R)对体外培养人IEC的损伤作用。结果表明:A/R可使IEC细胞存活率下降和乳酸脱氨酶(LDH)漏出增加,二者呈显著的负相关,其变化程度与A/R时间有依从关系。在缺氧前或缺氧后复氧前联合应用超氧化物歧化酶和过氧化氢酶可减轻A/R诱发的细胞存活率下降和LDH漏出量增加,应用别嘌呤醇世有同样的保护作用。表明:人IEC在复氧时可产生超氧基并引起细胞损伤。
Human primary cultured human intestinal epithelial cells (IEC) were used to observe the effect of hypoxia-reoxygenation (A / R) on human IEC cultured in vitro. The results showed that A / R could decrease the survival rate of IEC cells and increase the leakage of lactate deaminase (LDH). There was a significant negative correlation between the two. The degree of change was related to the A / R time. The combination of superoxide dismutase and catalase before anoxia or reoxygenation after hypoxia can reduce the decrease of cell viability induced by A / R and the increase of leakage of LDH. Allopurinol has the same protective effect . It shows that human IEC can produce superoxide and cause cell damage when reoxygenated.