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目的观察珍珠菜提取物(ZE4)对肝癌体内外的抑制作用。方法体外采用MTT法、3H-TdR掺入法、集落形成实验观察ZE4对肝癌SMMC-7721细胞的杀伤作用;采用划痕实验法与内皮细胞黏附实验观察ZE4对SMMC-7721细胞迁移的抑制作用。体内以抑瘤率和脏器指数为指标观察ZE4对小鼠肝癌H22的作用。结果ZE4可抑制SMMC-7721细胞的增殖和转移;体内实验显示ZE4可显著抑制小鼠肝癌H22的增殖,抑瘤率均在30%以上,其中中剂量(200mg/kg)组的效果最好,可达到57.8%。结论ZE4对肝癌有很好的抑制作用。
Objective To observe the inhibitory effect of Lysimachia chinesis extract (ZE4) on liver cancer in vitro and in vivo. Methods The killing effect of ZE4 on hepatoma SMMC-7721 cells was observed by MTT method, 3H-TdR incorporation method and colony formation assay. The inhibitory effect of ZE4 on the migration of SMMC-7721 cells was observed by scratch test and endothelial cell adhesion experiment. The effect of ZE4 on hepatoma H22 in mice was observed with the tumor inhibition rate and organ index in vivo. RESULTS: ZE4 inhibited the proliferation and metastasis of SMMC-7721 cells. In vivo experiments showed that ZE4 significantly inhibited the proliferation of hepatoma H22 in mice. The tumor inhibition rates were all above 30%. The medium dose (200 mg/kg) group had the best effect. It can reach 57.8%. Conclusion ZE4 has a good inhibitory effect on liver cancer.