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本文研究子宫刺激药与离体子宫组织合成前列腺素的相互关系。用器官溢流法检查子宫肌和子宫内膜对药物反应的差别。作者利用体重200～250g阉割后的雌性大鼠,于术后24小时开始使用17-β雌二醇(1mg/天,共3天),第四天将鼠用颈脱臼法处死。取出子宫立即浸入pH7.4冷Eagle培养基中,分离两子宫角并剔除脂肪和血管,每只子宫角两端插入清洁的乙烯管并用丝线结扎固定。组织水浴槽的温度调至37℃,用含有湿润的95%O_2和5%CO_2的液体溢流(superfuse),用另1导管与子宫角腹膜面上端接触,溢流液以0.2ml/分速度连续喷入于宫腔及其表面,分别收集其溢流液,样品未分析前应保
This article studies the relationship between uterine stimulants and prostaglandin synthesis in vitro. Uterine muscle and endometrial examination using organ overflow method of drug response differences. The authors used castrated female rats weighing 200-250 g and started 17-beta estradiol (1 mg / day for 3 days) 24 hours after surgery. On the fourth day, the rats were sacrificed by cervical dislocation. The uterus was removed immediately immersed in cold Eagle’s medium pH 7.4, the two horns were separated and fat and blood vessels were removed. A clean vinyl tube was inserted into each end of the uterine horn and ligated with silk. Tissue water bath temperature was adjusted to 37 ° C, with a wet 95% O_2 and 5% CO_2 liquid superfuse, another catheter with the uterine horn peritoneal surface contact, the overflow liquid at a rate of 0.2ml / min Continuous injection into the uterine cavity and its surface, respectively, to collect the overflow fluid, the sample should be analyzed before analysis