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目的 研究人大肠癌肝转移肿瘤中端粒酶活性和p16基因纯合缺失及其临床意义。方法 采用TRAP银染方法和半定量多重PCR检测 2 4例大肠癌肝转移肿瘤组织中端粒酶活性及p16基因的纯合缺失情况 ,并结合临床病理参数 ,进行统计学分析。结果 本组 2 4例大肠癌肝转移肿瘤组织中检测到 19例端粒酶阳性 ,阳性率为 79 2 %。端粒酶活性与转移瘤大小、肝内转移灶数目、分化程度、HBsAg以及是否伴有肝纤维化无关。在 2 4例转移瘤组织中有 9例标本中检测到p16基因的缺失 ,阳性率为 37 5 % ,p16基因的缺失与端粒酶活性相关。结论 大肠癌肝转移肿瘤中端粒酶活性和p16基因异常对阐明大肠癌转移的生物学行为有重要的意义。
Objective To study the telomerase activity and homozygous deletion of p16 gene in human colon cancer liver metastasis and its clinical significance. Methods TRAP silver staining method and semi-quantitative multiplex PCR were used to detect the telomerase activity and homozygous deletion of p16 gene in 24 cases of hepatic metastasis tumors of colorectal carcinoma, and the clinical pathological parameters were used for statistical analysis. Results Twenty-nine patients with colorectal cancer liver metastases had detected 19 cases of telomerase positive, the positive rate was 79. 2%. Telomerase activity was not associated with the size of metastases, number of intrahepatic metastases, degree of differentiation, HBsAg, and whether accompanied by liver fibrosis. The deletion of p16 gene was detected in 9 of 24 specimens of metastatic tumors. The positive rate was 37.5%. The deletion of p16 gene correlated with telomerase activity. Conclusion The abnormality of telomerase activity and p16 gene in colorectal cancer liver metastasis are important for elucidating the biological behavior of colorectal cancer metastasis.