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目的 探讨食管癌中 MT3基因 Cp G岛甲基化的情况及其临床意义。方法 选取切除的食管鳞癌标本4 7例 ,提取肿瘤、上切缘、下切缘及转移淋巴结的标本蜡块 DNA,经重亚硫酸化处理后 ,进行 COBRA分析 ,所得结果与各临床病理参数及随访资料进行比较。结果 全组有 10例 (2 1.3% )肿瘤组织发现 MT3基因甲基化 ,其中 1例呈原位癌样改变的上切缘也检测到甲基化。全组 35枚转移淋巴结中共发现 15枚 (4 2 .9% )甲基化 ,与肿瘤组织相比 ,差异有显著性 (P=0 .0 37,χ2 =4 .35 5 )。原发肿瘤存在甲基化 10例患者中 ,每例有 (5 .7± 3.6 0 )枚转移淋巴结。而不存在甲基化的 37例转移淋巴结数为 (3.2± 2 .13)枚 ,两者比较差异有显著性 (P=0 .0 18)。肿瘤组织的甲基化情况与患者的性别、年龄、烟酒嗜好、肿瘤分期及分化程度均无关 (P值均 >0 .0 5 ,χ2 检验 )。结论 MT3基因Cp G岛甲基化与食管鳞癌的淋巴结转移状况关系密切 ,作为一个独立的预后因素 ,在食管癌的发生和发展过程中发挥着一定作用
Objective To investigate the methylation of CpG island of MT3 gene in esophageal cancer and its clinical significance. Methods Forty-seven specimens of esophageal squamous cell carcinoma (ESCC) were selected for DNA extraction from the tumor, upper margin, lower margin and lymph node metastasis. COBRA analysis was performed after bisulfite treatment. The results were correlated with clinicopathological parameters, Follow-up data for comparison. Results The methylation of MT3 gene was found in 10 cases (21.3%) of the tumors in the whole group. One case was also methylated on the upper edge of the carcinoma in situ. A total of 15 (42.9%) methylation were found in 35 metastatic lymph nodes in all groups, which was significantly different from that in tumor tissues (P = 0.037, χ2 = 4.355). There were (5 .7 ± 3.60) metastatic lymph nodes in each of the 10 patients with methylation of the primary tumor. The number of metastatic lymph nodes in 37 cases without methylation was (3.2 ± 2.13) pieces, the difference was significant (P = 0.018). The methylation status of tumor tissue was not related to the patient’s gender, age, alcohol and tobacco preferences, tumor stage and degree of differentiation (P> 0.05, χ2 test). Conclusion Methylation of CpG island of MT3 gene is closely related to lymph node metastasis of esophageal squamous cell carcinoma and plays an important role in the occurrence and development of esophageal cancer as an independent prognostic factor