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目的:探讨胸腺基质淋巴生成素(thymic stromal lymphopoietin,TSLP)在肺癌组织中的表达及其与临床指标以及局部调节性T细胞(Regulatory T cells,Tregs)数量的相关性。方法:分别采用Q-RT-PCR和免疫组织化学染色方法检测TSLP在不同病变类型的肺组织中的表达,分析TSLP在不同病变肺组织中的表达差异;并运用免疫组化方法检测TSLP蛋白在不同病理类型的肺癌组织中的表达,分析TSLP表达与临床病理特征之间的相关性;采用免疫组化法检测肺癌组织中的Tregs细胞,分析TSLP表达与肿瘤局部Tregs细胞数量之间的关系。结果:TSLP基因在肺癌组织、癌旁组织、非肿瘤肺上皮均表达阳性,且表达差异无统计学意义;TSLP蛋白表达于胞浆中,其在肺癌组织中的阳性表达率(69.57%)显著高于肺的良性病变(13.33%)和非肿瘤肺上皮(30.00%),且TSLP表达与肿瘤大小和淋巴结转移有关;TSLP表达阳性的患者其肿瘤局部浸润的调节性T细胞数量明显高于TSLP阴性组(P<0.05)。结论:TSLP蛋白在肺癌组织中表达增加,且与肿瘤局部调节性T细胞数量增多有关,这提示TSLP可能是通过诱导Tregs增加在肺癌免疫耐受中发挥作用。
Objective: To investigate the expression of thymic stromal lymphopoietin (TSLP) in lung cancer tissues and its correlation with clinical parameters and number of regulatory T cells (Tregs). Methods: The expression of TSLP in lung tissues of different pathological types was detected by Q-RT-PCR and immunohistochemical staining, respectively. The expression of TSLP in different pathological changes of lung tissues was analyzed. The immunohistochemical method was used to detect TSLP protein in the lungs. Expression in different pathological types of lung cancer tissues was analyzed to analyze the correlation between TSLP expression and clinicopathological features. Immunohistochemistry was used to detect Tregs in lung cancer tissues and the relationship between TSLP expression and the number of tumor local Tregs was analyzed. Results: The expression of TSLP gene was positive in lung cancer tissues, adjacent tissues and non-tumorous lung epithelium, and there was no significant difference in expression. TSLP protein was expressed in cytoplasm and its positive expression rate in lung cancer tissues (69.57%) was significant. Higher than lung benign lesions (13.33%) and non-tumorous lung epithelium (30.00%), and TSLP expression was associated with tumor size and lymph node metastasis; TSLP-positive patients had significantly higher Treg cell infiltrations than TSLP. Negative group (P<0.05). Conclusion: The expression of TSLP protein in lung cancer is increased, and it is related to the increased number of regulatory T cells in the tumor. This suggests that TSLP may play an important role in the immune tolerance of lung cancer by inducing the increase of Tregs.