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将IL-2或/和IFN-γ基因体内转染腹腔巨噬细胞(MΦ),其表达的基因产物能提高MΦ的细胞毒活性,并诱导MΦ分泌TNF、IL-1和NO等抗肿瘤免疫介质.激发机体的非特异性免疫功能而产生抗肿瘤效应,特别是IL-2和IFN-γ基因联合转染MΦ,其表达的基因产物既能使MΦ产生更强的细胞毒活性并分泌高水平的TNF、IL-1和NO,又能激活脾CTL细胞,从而激发机体的非特异性和特异性免疫功能,产生更强的快同抗肿瘤效应.结果表明,IL-2和IFN-γ基因转染MΦ较单基因转染具有更强的抗瘤效应
The IL-2 or/and IFN-γ gene was transfected into peritoneal macrophages (MΦ) in vivo. The expressed gene product can increase the cytotoxic activity of MΦ and induce MΦ to secrete antitumor immunity such as TNF, IL-1 and NO. Medium. Inspires the body’s non-specific immune function to produce anti-tumor effects, especially when IL-2 and IFN-γ genes are combined to transfect MΦ. The expressed gene product can not only make MΦ produce stronger cytotoxic activity and secrete high levels of TNF, IL-1, and NO can activate spleen CTL cells, thereby stimulating the body’s non-specific and specific immune function, resulting in a stronger and faster anti-tumor effect. The results show that IL-2 and IFN-γ gene transfer Dyeing MΦ has a stronger anti-tumor effect than single gene transfection