目的了解珠海地区蚊媒携带流行性乙型脑炎(乙脑)病毒情况,为控制乙脑在人群中的流行提供依据。方法采集成蚊进行鉴定,并对蚊悬液采用细胞培养、逆转录聚合酶链反应(RT-PCR)以及荧光定量PCR技术进行病毒鉴定,并进行克隆与序列测定。结果2005年全年在养猪场共捕获成蚊876只,经鉴定隶属4属8种,其中以三带喙库蚊(35.73%)、致倦库蚊(32.54%)和海滨库蚊(21.35%)为主。在珠海各口岸捕获的542只蚊样本中,则以白纹伊蚊为主,占82.47%,其次为致倦库蚊,占16.25%。应用荧光定量PCR技术,在珠海地区注册养猪场和口岸的蚊媒中检出1份乙脑病毒阳性样本,但强度较弱,这与珠海地区是乙脑低发地区相一致。结论珠海地区存在乙脑的主要传播媒介,并且在蚊媒中携带有乙脑病毒,提示不能放松对乙脑的防控工作。 Objective To understand the situation of mosquito-borne Japanese encephalitis (JE) virus in Zhuhai and provide a basis for controlling the epidemic of JE in the population. Methods The adult mosquitoes were collected and identified. The virus was identified by cell culture, reverse transcription polymerase chain reaction (RT-PCR) and fluorescence quantitative PCR (PCR). The cloned and sequenced strains were also identified. Results A total of 876 adult mosquitoes were captured in pig farms in 2005, of which 8 were identified in 4 genera. Among them, Culex tritaeniorhynchus (35.73%), Culex pipiens pallets (32.54%) and Cucurbitacide septa %) Based. Among the 542 mosquito samples captured in all ports of Zhuhai, Aedes albopictus was the dominant species, accounting for 82.47%, followed by Culex quinquefasciatus, accounting for 16.25%. Using fluorescence quantitative PCR, one positive sample of JE virus was detected in the mosquito media of registered pig farms and ports in Zhuhai, but its intensity was weaker, which was consistent with the low incidence of JE in Zhuhai. Conclusion There is a major transmission vector for JE in Zhuhai area and JE virus is carried in mosquito vectors, suggesting that we can not relax the prevention and control of JE.