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本文作者通过孕妇羊水中氯化乙酰酯酶(AChE)的活性和AFP浓度的测定,去确定染色体异常及神经管缺损的胎儿,作为出生前的筛选试验。被检对象为30个正常孕妇、12个有神经管缺损胎儿的孕妇,9个经羊水细胞培养确定为染色体异常将羊水进行AFP浓度和AChE活性测定。AFP的浓度使用聚乙二醇沉淀单抗体放射免疫分析,AChE活性是在Cobas离心分析器中30℃的反应率来测定的。结果表明,在所有7例21三体和单独1例3部份三体孕妇的羊水中,AFP浓度显著地低于相应孕期正常妇女的范围,而AChE活性在相应的孕期中均在正常范围内。单独1例13三体AFP浓度和
Through the determination of the AChE activity and the concentration of AFP in the amniotic fluid of pregnant women, the authors determined the fetuses with chromosomal abnormalities and neural tube defects as screening tests before birth. The subjects were 30 normal pregnant women, 12 pregnant women with neural tube defects fetuses, 9 amniotic fluid cells identified as chromosomal abnormalities amniotic fluid AFP concentration and AChE activity was measured. AFP concentrations were determined using polyethylene glycol precipitation single antibody radioimmunoassay and AChE activity was measured at 30 ° C in a Cobas centrifuge. The results showed that the AFP concentration in amniotic fluid of all seven trisomy 21 and one tripartite trisomy 3 was significantly lower than that of normal women during the corresponding trimester, whereas AChE activity was within the normal range for the corresponding trimester . One case of 13 trisomy AFP concentration alone