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目的构建pcDNA_3/p16真核表达质粒并了解其对肝癌细胞BEL-7404生长的抑制作用。方法将p16 cDNA亚克隆至pcDNA_3真核表达载体上,并经脂质体介导转染至BEL-7404细胞中。应用MTT法和流式细胞仪分析转染细胞生长情况和细胞周期。结果重组pcDNA_3/p16表达质粒构建成功,经pcDNA_3/p16转染的BEL-7404细胞生长速度受到明显抑制,且细胞多停滞于G_0/G_1期。结论重组pcDNA_3/p16质粒能在BEL-7404细胞内表达,且能抑制BEL-7404细胞的生长。
Objective To construct pcDNA_3/p16 eukaryotic expression plasmid and to understand its inhibitory effect on the growth of hepatocellular carcinoma cell line BEL-7404. Methods p16 cDNA was subcloned into pcDNA_3 eukaryotic expression vector and transfected into BEL-7404 cells via liposome. MTT assay and flow cytometry were used to analyze the growth and cell cycle of transfected cells. Results The recombinant pcDNA_3/p16 expression plasmid was successfully constructed. The growth rate of BEL-7404 cells transfected with pcDNA_3/p16 was significantly inhibited, and cells were mostly arrested in the G_0/G_1 phase. Conclusion The recombinant plasmid pcDNA_3/p16 can be expressed in BEL-7404 cells and can inhibit the growth of BEL-7404 cells.