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目的研究壳聚糖絮凝沉降法对延胡索水提液的纯化效果。方法以延胡索乙素和浸膏率为纯化考察指标,采用L(934)正交试验设计,并结合指纹图谱,优选壳聚糖絮凝沉降法的最佳纯化工艺。结果壳聚糖絮凝沉降法纯化延胡索水提液的最佳条件是药液质量浓度为0.25 g.mL-1,静置温度为5℃,不调pH值,加入药液体积20%的壳聚糖溶液。结论壳聚糖澄清剂可用于延胡索水提液的纯化。
Objective To study the purification effect of Chitosan flocculation settling method on Corydalis yanhusuo aqueous extract. Methods Tetrahydropalmatine and its extract rate were used as purification indexes. The orthogonal design of L (934) was used, and the best purification technology of chitosan flocculation and sedimentation method was optimized. Results Chitosan flocculation sedimentation method to purify the aqueous extract of Corydalis best conditions are liquid mass concentration of 0.25 g.mL-1, standing temperature of 5 ° C, do not adjust the pH value, adding liquid volume 20% of the shell poly Sugar solution. Conclusion Chitosan clarifier can be used to purify the aqueous extract of Corydalis.