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以白三叶草种子为试材,用ACC9-1脱氨酶促生菌的菌体悬浮液对白三叶草种子进行浸泡处理并用夹板法种植于不同浓度氯化钠胁迫的溶液中(水培),检测了不同处理下白三叶草的生理、生化指标,比较了促生菌对白三叶草生长的影响。结果表明:随着氯化钠处理浓度的升高,白三叶草幼苗受到的胁迫程度不断增加,当加入促生菌时,胁迫程度降低。说明植物根际促生菌的加入对白三叶草幼苗的盐害有缓冲、调节和适应的作用,因而对降低白三叶草幼苗盐害有重要作用。
Using white clover seeds as test material, the white clover seeds were immersed in the bacterial suspension of ACC9-1 deaminase-producing bacteria and were planted in solution with different concentrations of sodium chloride stress (Hydroponics) The physiological and biochemical indexes of white clover under different treatments were compared, and the effects of growth promoting bacteria on the growth of white clover were compared. The results showed that with the increase of sodium chloride concentration, the stress level of white clover seedlings increased continually, and the degree of stress decreased when adding the growth promoting bacteria. The results showed that the addition of plant growth promoting bacteria had the effect of buffering, regulating and adapting to the salt damage of white clover seedlings and therefore played an important role in reducing salt damage of white clover seedlings.