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目的探讨雄激素对THP-1巨噬细胞源性泡沫细胞胆固醇含量的影响。方法 0.4μmol/L睾酮处理THP-1巨噬细胞源性泡沫3、6、12和24h以及不同浓度的睾酮处理THP-1巨噬细胞源性泡沫24h,采用高效液相色谱检测细胞内总胆固醇和游离胆固醇的含量。结果 0.4μmol/L睾酮作用12和24h其细胞内总胆固醇、游离胆固醇和胆固醇酯明显增加,与各自对照组比较差异有统计学意义(P<0.05);0.4μmol/L和4μmol/L睾酮处理24h组其细胞内总胆固醇、游离胆固醇和胆固醇酯明显增加,与对照组比较差异有统计学意义(P<0.05)。结论超生理浓度的雄激素可能通过影响细胞胆固醇含量而参与和/或促进AS的发生发展。
Objective To investigate the effect of androgens on the cholesterol content of THP-1 macrophage-derived foam cells. Methods THP-1 macrophage-derived foam was treated with 0.4μmol / L testosterone for 3, 6, 12 and 24h, and different concentrations of testosterone were used to treat THP-1 macrophage-derived foam for 24 hours. High performance liquid chromatography And free cholesterol content. Results The levels of total cholesterol, free cholesterol and cholesteryl ester in test group increased significantly at 12 and 24 h after treatment with 0.4 μmol / L testosterone (P <0.05), while those with 0.4 μmol / L and 4 μmol / L testosterone The levels of total cholesterol, free cholesterol and cholesteryl ester in 24h group were significantly increased compared with the control group (P <0.05). Conclusion Superspecific concentrations of androgens may participate in and / or promote the development of AS by affecting the cellular cholesterol content.