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目的观察细胞株SKOV3/si RNA Her-2基因的沉默作用及生物学效应。方法分别将SKOV3/si RNA及对照组SKOV3种植NOD-SCI D小鼠皮下,监测两组瘤体积,3个月后处死NOD-SCI D小鼠,称瘤质量,并采用RT-PCR方法鉴定抑制Her-2表达的效果。结果建立的抑制Her-2基因表达的SKOV3细胞株(SKOV3/si RNA)成瘤力下降。结论动物实验证实RNAi沉默Her-2基因可影响卵巢癌的成瘤力,RNAi技术有可能成为治疗卵巢癌的新途径。
Objective To observe the silencing effect and biological effect of SKOV3 / si RNA Her-2 gene. METHODS: SKOV3 / si RNA and control SKOV3 were respectively subcutaneously implanted into NOD-SCI D mice to monitor the tumor volumes. After 3 months, NOD-SCI D mice were sacrificed and the tumor mass was measured. The inhibition was determined by RT-PCR The effect of Her-2 expression. Results The SKOV3 cell line (SKOV3 / si RNA), which inhibits the expression of Her-2 gene, had a reduced oncosis potential. Conclusion Animal experiments confirmed that RNAi silencing Her-2 gene can affect the tumorigenicity of ovarian cancer, RNAi technology may be a new way to treat ovarian cancer.