论文部分内容阅读
利用新色原建立测定血清葡萄糖浓度的方法．以葡萄糖氧化酶为基础用双试剂结合速率法进行定量分析．结果：葡萄糖浓度达50．0mmol／L反应仍呈良好的线性；回收率为99．9％，日内CV为0．88％，日间CV为2．03％，除高浓度的血红蛋白外，能有效地避免血清中氧化物、浊度、色素等物质的干扰，测定结果与葡萄糖氧化酶／酚法和葡萄糖激酶法有良好的相关性，参考值范围为3．88～5．56（4．72±0．43）mmol／L．结论：该方法由于试剂稳定，成本低，测定方法可靠，适合在具有双试剂分析功能的自动生化分析仪上使用．
The method of determining serum glucose concentration by using new chromogen was established. On the basis of glucose oxidase, two reagents and rate method were used for quantitative analysis. Results: The glucose concentration was 50.0mmol / L, the reaction was still good linearity; the recovery rate was 99.9%, intraday CV was 0.88%, intraday CV was 2.03%, in addition to high concentrations of hemoglobin, Effectively avoid the serum oxide, turbidity, pigment and other substances interfere with the determination results and glucose oxidase / phenol method and glucokinase method has a good correlation, the reference value range of 3.88 ~ 5.56 (4. 72 ± 0.43) mmol / L. Conclusion: This method is suitable for automatic biochemical analyzer with dual reagent analysis because of its stable reagent, low cost and reliable assay.