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经沼液浸种的早稻秧苗比对照组秧苗，具有较强的耐冷性，通过电镜对叶肉细胞超微结构动态观察证实，在低温（５°／５℃）下，叶绿体结构和细胞核结构，随着冷害天数的延长，伤害在不断加重，叶绿体结构会出现空洞，基质片断裂，直至崩溃。细胞核染色体凝集，核膜破裂，核内容物丧失等现象出现。而经沼液浸种的秧苗伤害程度有较明显的减轻。
The seedlings of early rice soaked with biogas slurry had stronger cold tolerance than the control group. Dynamic observation of the ultrastructure of mesophyll cells by electron microscopy confirmed that at low temperature (5 ° / 5 ° C), the chloroplast structure and nucleus structure changed with Chilling injury days, the damage is increasing, there will be hollow chloroplast structure, matrix piece rupture, until the collapse. Nuclear chromatin agglutination, nuclear membrane rupture, nuclear content loss and other phenomena appear. However, the degree of damage to seedlings soaked in biogas slurry was significantly reduced.