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目的:脾脏在小鼠前房相关免疫偏离(anterior chamber-associated immune deviation,ACAID)的诱导中起着重要的作用。在前房接种抗原4天内摘除脾脏可以消除免疫偏离而导致免疫反应。本研究旨在观察脾脏在其他种类动物ACAID的诱导和维持中的作用。方法:在脾脏切除和假性脾脏切除后,使用牛血清白蛋白(bovine serum albumin,BSA)作为可溶性抗原分别接种于Wistar大鼠、新西兰白兔和猕猴的眼前房。使用BSA和完全弗氏佐剂混合乳剂免疫动物。通过抗原刺激皮肤测定抗原特异性迟发型超敏反应(delayed-type hypersensitivity,DTH),并定时评估免疫偏离的维持时间。结果:假性脾脏切除眼内抗原接种组和脾脏切除眼内抗原接种组所有动物均显示抗原特异性DTH抑制,且维持时间无显著差异。相反,单纯假脾脏切除组和单纯脾脏切除组动物均显示阳性抗原特异性DTH反应。结论:脾脏对于大鼠、兔和灵长目动物ACAID的诱导和维持不具有重要作用。这些发现提示随着进化程度的进阶,脾脏的功能可能被其他的淋巴器官所代替。眼科学报1999;15:221-224。
Objective: Spleen plays an important role in the induction of anterior chamber-associated immune deviation (ACAID) in mice. Vaccination of the spleen in the anterior chamber with the antigen within 4 days can eliminate the immune bias and lead to an immune response. This study aimed to observe the role of the spleen in the induction and maintenance of ACAID in other species of animals. METHODS: After splenectomy and pseudo-splenectomy, bovine serum albumin (BSA) was used as soluble antigen in the anterior chamber of Wistar rats, New Zealand white rabbits and macaques, respectively. Animals were immunized with BSA and complete Freund’s adjuvant emulsion. Antigen-specific delayed-type hypersensitivity (DTH) was measured by antigen-stimulating the skin and the maintenance time of immune deviation was periodically assessed. Results: All animals in the spleen resection intraocular antigen inoculation group and the splenectomy intraocular antigen inoculation group showed antigen-specific DTH inhibition with no significant difference in maintenance time. In contrast, both the group of pure spleen resection and the group of simple spleen resection showed positive antigen-specific DTH reaction. Conclusion: The spleen does not play an important role in induction and maintenance of ACAID in rats, rabbits and primates. These findings suggest that spleen function may be replaced by other lymphoid organs as the degree of evolution is advanced. Journal of Ophthalmology 1999; 15: 221-224.