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在 M_8诱导培养基申附加适当的2,4-D、NAA、Kt、6-BA 和4pu-30等激素,对籼粳、籼籼交水稻 F_1的花药进行培养。结果表明,在8种处理中,以2.4-D 2mg/L 加 NAA 2mg/L、Kt 1mg/L、6-BA 0.5mg/L 对籼粳交材料平均愈伤组织(7.7%)虽略低于其它5个处理(8.94%～11.88%),但绿苗分化率最高(17.74%)。对籼籼交水稻的出愈率(0.61%)虽低于其它两个处理(13.5%～15.3%),但绿苗分化率(50%)和花药培养力(0.3%)都明显超过其它处理(分别为0～7.69%和0～0.1%)。2,4-D 与 NAA 配合附加不同的细胞分裂素分别比单独用2,4-D 或2,4-D配 Kt 的处理的花药培养力高.附加激动素对愈伤组织的发生有抑制作用,但能提高绿苗分化率.6-BA 与激动素的作用相似。4pu-30与2,4-D、NAA 配合,有利于愈伤组织的产生,但有降低其质量的趋势,且与其它细胞分裂数有拮抗作用。
Anther of indica-japonica rice and indica-indica hybrid rice F_1 was cultured with appropriate 2,4-D, NAA, Kt, 6-BA and 4pu-30 in M_8 induction medium. The results showed that the average callus (7.7%) of indica japonica hybrid rice was slightly lower with 2.4-D 2mg / L plus NAA 2mg / L, Kt 1mg / L and 6-BA 0.5mg / L The other five treatments (8.94% ~ 11.88%), but the highest rate of green seedlings (17.74%). Although the yield of indica indica hybrid rice (0.61%) was lower than that of the other two treatments (13.5% ~ 15.3%), the differentiation rate of green plantlets (50%) and anther culture ability (0.3%) were significantly higher than those of other treatments (0 to 7.69% and 0 to 0.1%, respectively). Addition of 2,4-D to NAA with different cytokinin concentrations was higher than that of anther treated with 2, 4-D or 2, 4-D with Kt alone.Additional kinetin inhibited the occurrence of callus But increased the rate of green shoot differentiation.6-BA has similar effects to kinetin. 4pu-30 with 2,4-D, NAA, is conducive to the generation of callus, but there is a tendency to reduce its quality, and with other cell division number antagonism.