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本文用反相高效液相色谱法测定了利福定在人体尿液中的浓度。色谱条件为:Shimpack CLC-TMS 5μm柱(150×4.6mm);0.01M磷酸二氢钾缓冲液—乙腈(50:50)为流动相;检测波长316nm.尿中利福定在0.2～20μg/ml的范围内呈线性关系。有机溶媒提取率为93%.用本法测定了利福定固体分散物相对于原药的生物利用度为147.7%.
In this paper, the determination of rifadin in human urine by reversed-phase high performance liquid chromatography. Chromatographic conditions: Shimpack CLC-TMS 5μm column (150 × 4.6mm); 0.01M potassium dihydrogen phosphate buffer - acetonitrile (50:50) as the mobile phase; detection wavelength of 316nm, urine rifadin 0.2 ~ 20μg / ml in the range of a linear relationship. The organic solvent extraction rate was 93% .The bioavailability of the solid dispersion of rifabadin relative to the original drug was determined by this method to be 147.7%.