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本文目的是用污泥直接接种法与另外三种不同的检测法(牛肉浸液洗脱法,酸沉淀后牛肉浸液洗脱法,明矾沉淀后Tris缓冲液洗脱法)检测,消化的和未经处理的污泥的病毒含量,以资比较。污泥样品采自厌氧消化的三个不同的处理厂,及以污泥作肥料的现场。采样量为1升,于4℃过夜,次日进行检测。病毒检测是采用Hela细胞培养,每个样品接种四支细胞管,阴性培养物进行盲目传代两次。原接种物如为阳性,则用Hank′s液进行连续10倍稀释,每个稀释度接种三支细胞
The purpose of this paper is to use the direct inoculation of sludge with three different detection methods (beef extract, beef extract after acid precipitation elution, alum precipitated Tris buffer elution method) to detect and digest Untreated sludge virus content, to compare. Sludge samples were collected from three different treatment plants for anaerobic digestion and were treated with sludge as fertilizer. The sample volume was 1 liter, overnight at 4 ° C and the next day for testing. Virus detection was performed using Hela cells, with four tubes per sample seeded and negative cultures blindly passaged twice. If the original inoculum is positive, a serial 10-fold dilution is made with Hank’s solution and three cells are seeded at each dilution