论文部分内容阅读
目的:建立以反相高效液相色谱法测定清肺合剂中东莨菪内酯含量的方法。方法:色谱柱为C18(250mm×4.6mm,5μm),流动相为甲醇-0.5%醋酸(40∶60),检测波长为345nm,流速为1.0mL·min-1。结果:东莨菪内酯进样量在0.04～0.80μg范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为98.6%,RSD=1.35%(n=6)。结论:本方法方便易行、结果准确、重复性好,可用于清肺合剂的质量控制。
Objective: To establish a method for the determination of scopoletin in Qingfei mixture by reversed-phase high performance liquid chromatography. METHODS: The chromatographic column was C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol-0.5% acetic acid (40:60). The detection wavelength was 345 nm and the flow rate was 1.0 mL · min-1. Results: The linear range of scopolactone was 0.04 ~ 0.80 μg with the peak area integral (r = 0.9999). The average recovery was 98.6% with RSD of 1.35% (n = 6). Conclusion: The method is convenient and easy to operate with accurate results and good reproducibility. It can be used for the quality control of Qingfei Mixture.