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将稻田土和病稻苗上分离的Fusariumequiseti,F.oxysporum,F.graminearum和Rhizoctoniasolani,接种在免疫育苗法和普通旱育苗法培育的秧苗上。免疫育苗的秧苗提高抗病力95.5%~97.8%。测定其秧苗接种病原菌后防御酶系的动态表明:1.过氧化物酶活性峰比对照提前36h,且峰值高;2.超氧化物歧化酶活峰比对照提前4h;3.苯丙氨酸解氨酶的活力比对照高1倍多。同时免疫育苗秧苗新陈代谢旺盛、生理活性高。表现在:1.叶片叶绿素含量高(3.12mg/ml)、光合作用强度大(887molO2/g.h),因而干物质积累多;2.根系发达,单株根数比普通旱育秧苗多3.9条/株;3.根系色白有弹性,活力强,对α-萘胺氧化力达60μg/g.h,提高30%;4.束缚水含量达64.89%,显著高于普通旱育苗;5.根冠比比普通旱育苗高28%。
Fusariumequiseti, F oxysporum, F. graminearum and Rhizoctoniasolani were inoculated on seedlings nurtured by the immunization nursery method and ordinary drought nursery method. Seedlings of immune nursery raise disease resistance 95.5% ~ 97.8%. The dynamics of the defensive enzymes after the seedlings were inoculated with the pathogen showed that: 1. Peroxidase activity peak 36h earlier than the control, and the peak is high; 2. Survival peak of superoxide dismutase 4h ahead of the control; 3. Phenylalanine ammonia lyase activity than control more than doubled. At the same time immune nursery seedlings strong metabolism, high physiological activity. Reflected in: 1. Leaf chlorophyll content (3.12mg / ml), photosynthesis intensity (887molO2 / g.h), and thus more dry matter accumulation; Roots developed, the number of roots per plant more than ordinary dry-seedlings 3.9 / plant; 3. Root color white flexible, strong vitality, α-naphthylamine oxidation of 60μg / g. h, increased by 30%; 4. Bound water content of 64.89%, significantly higher than ordinary drought nursery stock; Roots crown than ordinary dry nursery 28% higher.