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目的:研究女性生殖道感染高危型人乳头瘤病毒(high rist human papillomavirus,HR-HPV)与宫颈病变存在的相关性。方法:收集近3万例已婚妇女宫颈外口及宫颈管分泌物,采用针对13种高危型HPV DNA序列的特异性引物,结合特异性的TaqMan荧光标记探针技术通过实时荧光PCR仪,进行同步核酸扩增与检测,高危阳性者建议行阴道镜检测,有病变者进行活组织检查,探讨HR-HPV与宫颈病变的相关性。结果:3万例妇女体检中查出HR-HPV感染阳性率为6.35%,其中175例阳性妇女行阴道镜和活组织检查,其中宫颈癌6例(3.4%),宫颈上皮内瘤样I级8例(4.6%),II级9例(5.1%),III级28例(16%)。结论:HR-HPV检测是子宫颈疾病筛查的有效手段,是子宫颈癌及癌前病变发病的主要危险因素。
Objective: To investigate the relationship between high-risk human papillomavirus (HR-HPV) and cervical lesions in female reproductive tract infections. Methods: Nearly 30 000 married women were collected cervical cervix and cervix secretion, using specific primers for 13 kinds of high-risk HPV DNA sequences, combined with specific TaqMan fluorescent labeling probe technology by real-time fluorescence PCR instrument Synchronous nucleic acid amplification and detection, high-risk positive line recommended colposcopy, patients with lesions biopsy to explore HR-HPV and cervical lesions. Results: The positive rate of HR-HPV infection detected in 30,000 women was 6.35%. Among them, 175 cases were positive for colposcopy and biopsy, including 6 cases of cervical cancer (3.4%), 1 case of cervical intraepithelial neoplasia 8 cases (4.6%), 9 cases of grade II (5.1%) and 28 cases (16%) of grade III. Conclusion: HR-HPV test is an effective method to screen cervical diseases and is the main risk factor of cervical cancer and precancerous lesions.