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实验方法应用健康的、曾生过一胎的,体重2～3kg 的家兔进行实验,随机分成两大组:眼前房移植卵巢家兔组和原位卵巢家兔组。前者在无菌条件下,将卵巢移植到眼前房内,移植成功后,用1%醋酸铜诱导排卵,凡是有排卵的家兔用来实验,又随机分成三小组,(1)雌二醇(E_2)组:连续三天单纯皮下注射 E_2100μg,每日每只家兔注射一次,处理前抽静脉血一次,第三次注射 E_2后的第2、4、6、19、72
Experimental methods Healthy rabbits with a birth weight of 2 to 3 kg were used to conduct experiments. They were randomly divided into two groups: anterior chamber transplantation of ovary rabbits and orthotopic ovarian rabbits. The former under the aseptic condition, the ovary transplanted into the anterior chamber of the eye, after the success of transplantation, ovulation induction with 1% copper acetate, any rabbits with ovulation for experiments, and was randomly divided into three groups, (1) estradiol ( E_2) group: E_2100 μg was injected subcutaneously in each subcutaneous injection for three consecutive days. Each rabbit was injected once a day. Before the treatment, venous blood was drawn once. The second 2, 4, 6, 19, and 72 after the third injection of E_2 were performed.