应用大片段ＰＣＲ对线粒体ＤＮＡ缺失的快速定位技术对线粒体肌病患者线粒体ＤＮＡ缺失的定位需要用不同限制性酶和不同ＤＮＡ探针进行Ｓｏｕｎｔｈｅｒｎ杂交分析。然后对这些限定的断裂点区域再进行短片段的ＰＣＲ扩增，从而进一步用于序列分析。上述方法的第一步很费时... Rapid localization of mitochondrial DNA using large fragment PCR for mapping mitochondrial DNA deletions in patients with mitochondrial myopathy requires Sounthern hybridization with different restriction enzymes and different DNA probes. A second PCR amplification of the short fragment was then performed on these defined breakpoint regions for further use in sequence analysis. The first step of the above method is time consuming ...