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目的:观察活血潜阳方对高血压大鼠内皮祖细胞及PI3K/Akt的影响。方法:WKY12只,SHR 36只,随机分为模型组,活血潜阳方组,代文组,灌胃8周。采用密度梯度离心法从高血压大鼠血中分离内皮祖细胞,收集培养5天的内皮祖细胞,鉴定、计数,采用Realtime-PCR和Western blot检测内皮祖细胞中PI3K、Akt mRNA和蛋白表达水平。结果:与模型组比较,活血潜阳方组和代文组循环内皮祖细胞数量均增高(P<0.01),且活血潜阳方组较代文组增高显著(P<0.05);与模型组比较,活血潜阳方组和代文组P13K、Akt mRNA和蛋白表达水平均增高(P<0.01或P<0.05),其中活血潜阳方组Akt蛋白较代文组增高显著(P<0.05)。结论:活血潜阳方可能通过调控PI3K/Akt信号通路,提高循环内皮祖细胞数量,从而保护血管内皮。
Objective: To observe the effect of Huoxue Qianyang Recipe on PI3K / Akt in hypertensive rats. Methods: WKY12 only, SHR 36, were randomly divided into model group, Huoxue Qianyang group, Daiwen group, gavage for 8 weeks. Endothelial progenitor cells were isolated from the blood of hypertensive rats by density gradient centrifugation. EPCs cultured for 5 days were collected, identified, and counted. The expression of PI3K and Akt mRNA and protein in endothelial progenitor cells were detected by Realtime-PCR and Western blot . Results: Compared with the model group, the number of circulating EPCs in Huoxue Qianyang group and Daiwen group increased (P <0.01), and the expression of Huoxue Qianyang group increased significantly (P <0.05) compared with model group (P <0.01 or P <0.05). The expression of Akt protein in Huoxue Qianyang group was significantly higher than that in the meridian group (P <0.05) . Conclusion: Huoxue Qianyang Prescription can protect the vascular endothelium by regulating the PI3K / Akt signaling pathway and increasing the number of circulating endothelial progenitor cells.